Electrospun PVA–Dextran Nanofibrous Scaffolds for Acceleration of Topical Wound Healing: Nanofiber Optimization, Characterization and In Vitro Assessment

Abstract Electrospun polyvinyl alcohol–dextran (PVA–Dex)-based nanofibers (NFs) are explored as a novel class of bioactive injury dressing materials, which have an essential role for topical mending. Sodium ampicillin-loaded citric acid-cross-linked PVA–Dex NFs were fabricated by electrospinner wound recuperating purposes. Results revealed that PVA (10%)–dextran (10%) cross-linked with 5% acid (CA) was chosen optimized condition obtaining non-beaded and morphological accepted nanofibers. Altered concentrations CA cross-linker progressively enhanced significantly the mechanical/thermal stability wettability-proof scaffolds, compared to un-cross-linked (PVA–Dex) scaffolds. Meanwhile, swelling (%), protein adsorption released ampicillin decreased dramatically increase in concentration, conversely increasing dextran concentrations. Interestingly, resultant high promoted proliferation HFB-4 cells concentration-dependent manner antimicrobial activity behavior, containing after 24 48 h cell exposure. Notably, all remarked ability accelerate rate vitro gap closure (%) treatment h, control sample. However, reducing concentration showed highest percentages healing scratched clear observed process.